BriteVu Enhancer is a non-toxic liquid and is added to the BriteVu and water mix.
BriteVu Enhancer significantly reduces micro aggregation that is associated with Van der Waals forces. These weak forces sometimes act to bring small particles (as are found with BriteVu) together. Enhancer significantly reduces micro clumps in the 15-40 µm range that can plug small vessels and capillaries and cause ‘bright spots’ on micro-CT.
BriteVu Enhancer better suspends and disperses the radiodense agent in the solution thereby reducing gravity dependent settling effect. This results in more even radiodensity across the vessel, or other perfused subject, diameter making segmentation and tracing easier.
BriteVu Enhancer lowers the temperature of solidification. This allows researchers to perfuse BriteVu at lower temperatures for better histologic preservation. Without Enhancer, BriteVu solidifies around 38-40 deg C. With Enhancer, BriteVu solidifies around 31-33 deg C. This feature also increases working time prior to solidification.
There is no need to use other suspending or surfactant agents, such as Dawn dish soap, when using BriteVu Enhancer.
BriteVu Enhancer is dosed at 1-2% (in ml volume) to BriteVu (in gram weight) prior to mixing with water or other solvents.
For example, if performing a whole body perfusion on a typical 20-25 g mouse via cardiac puncture, you will need about 15 g dry weight of BriteVu. This is regardless of water volume used. You will need 0.15-0.30 ml of BriteVu Enhancer.
Calculation: 15 g (dry BriteVu) X 0.01-0.02= 0.015-0.30 ml/cc (liquid BriteVu Enhancer)
For most studies, dose BriteVu Enhancer at 1.5% the BriteVu weight.
One bottle of BriteVu Enhancer is enough for 1.5-3 Kg of dry BriteVu.
- Heat calculated water (solvent) amount to 40-45 deg C.
- Add BriteVu Enhancer liquid (calculated based on dry BriteVu weight).
- Wait one minute and then add dry BriteVu to solution.
- Heat and continuously mix solution to 70-80 deg C for 10 minutes.
- Lower temperature to desired range and perfuse subject.
- Let perfused subject cool (ice water bath, refrigerator, etc) until BriteVu mixture solidifies and then CT scan. Alternatively, subject and/or its tissues can be stored in formalin for scanning later.
Item #: 040232270796
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